I have had a career as a basic scientist investigating the regulation of phospahtidylcholine (PC) biosynthesis. In the last decade the emphasis has changed to function of PC since we have been able to make several knockout mice in PC biosynthesis. We have found that impaired PC biosynthesis via the phosphatidylethanolamine N-methyltransferase (PEMT) results in liver disease. On the positive side, when mice lack PEMT, they are very much protected against atherosclerosis, obesity and diabetes. We have also studied mice that lack choline kinase beta, the first enzyme in PC biosynthesis via the choline pathway. These mice develop hindlimb muscular dystrophy and neonatal bone deformity. Our work will continue to explore these diseases and the role of PC biosynthesis.