Lipid and lipid metabolite analysis
Access support for measuring lipid-related compounds in experimental samples
Lipids are of particular importance in some of today’s most prevalent health issues. (Examples include: obesity, diabetes, nutrition, neuroscience, cardiovascular disease and others.)
This core facility is a project initiated and supported by the Group on the Molecular and Cell Biology of Lipids and WCHRI, with major support from FoMD. WCHRI members receive subsidized/discounted pricing for this service.
While the lipidomics core facility is constantly evolving to add new methods as needed, current services include:
- lipoprotein profiling from plasma, cultured cell medium, and intracellular compartments by fast protein lipid chromatography (FPLC).
- non-destructive separation and collection of lipoproteins (very low density lipoprotein (VLDL), low-density lipoprotein (LDL), high performance liquid chromatography (HDL) by fast protein liquid chromatography (FPLC).
- separation and measurement of alcohols, fatty acids, and total lipid classes by gas chromatography (GC).
- separation and measurement of bile acids, phospholipids, total lipid classes, resveratrol, and sulforaphane by high performance liquid chromatography (HPLC).
- measurement of phospholipids and sphingolipids by mass spectrometry (with Jonathan Curtis, AFNS).
The lipidomics core facility has also developed high resolution mass spectrometric methods for targeted and untargeted lipidomic analysis, and is working on validating the software analysis of these complex data sets.
Lipids currently measured include: Cholesterol, triglycerides, phospholipids, fatty acids and cholesteryl esters. Addition of the mass spectrometer will allow measurement of many other lipids, with much lower detection limits than previously possible.
This facility also offers customized chromatographic analyses based on the needs of individual users. Rates start at $15/sample.
Please feel free to email firstname.lastname@example.org if there is a technique you would like to see included in our services, or if you have any other questions.
For more information, visit the University of Alberta Lipidomics Core Facilities website.